M.Sc. Eng. Thesis Colloquium
Dear All,
DEPARTMENT OF INSTRUMENTATION AND APPLIED PHYSICS
M.Sc. Eng. Thesis Colloquium
NAME OF THE CANDIDATE : Mr. Jigmi Basumatary
DEGREE : M.Sc.Eng
TITLE OF THE THESIS : Light Illuminated Volume Expedite Imaging
(LIVE) system
SUPERVISORS : Prof. Partha P Mondal
DATE & TIME : Tuesday, 26th November, 2019 at 11.00 A.M.
VENUE : Seminar Hall, Dept. of Instrumentation and
Applied Physics.
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ABSTRACT
Optical fluorescence microscopy is one of the fundamental tools, that is essential for the study of complex and dynamic biological processes in 3D. Key issues associated with the existing imaging system are, photobleaching, long-time imaging and low-quality volume reconstruction. Continuous monitoring of large specimens for long durations requires fast volume imaging. This is essential for understanding processes occurring during developmental stages of multicellular organisms. One of the key obstacles for prolonged monitoring and data collection is photobleaching. To overcome the effect of bleaching, we developed single and multi-color Lightsheet Illuminated Volume Expedite (LIVE) imaging technique that enables rapid screening of multiple tissues in an organism with order-less photobleaching. Our approach based on LIVE imaging employs quantized step rotation of the specimen to record 2D angular data that reduces data collection time by a factor of > 10 when compared to existing light sheet microscopy. A co-planar multicolour light sheet is generated to excite spectrally-separated fluorescent probes that label the target tissues. Audrino-based control systems were employed to automatize and control the volume data acquisition process. To illustrate the advantages of our approach, we have noninvasively imaged Drosophila larvae (without removing chitinous) and Zebrafish embryo. Dynamic studies of multiple organs (muscle and yolk-sac) in Zebrafish over a prolonged duration of time (5 days) were carried out to understand muscle structuring and metabolism. Volume reconstruction, intensity plots and inter-dependence ratio analysis allowed us to understand the transition from lipid-based metabolism to protein-based metabolism during early development (Pharyngula period) in Zebrafish. The advantage of multi-color lightsheet illumination, fast volume imaging, simultaneous multiple organ imaging and order-less photobleaching makes LIVE imaging the system of choice for rapid monitoring and real-time assessment of macroscopic biological organisms at microscopic resolution.
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ALL ARE WELCOME
CHAIRMAN
Date/Time
Date(s) - 26/11/2019
11:00 am - 12:00 pm
Seminar Hall, Dept. of Instrumentation and Applied Physics
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