IN267: Fundamentals of Fluorescence Microscopy (Aug.-Dec.)

Abstract:

Light Sources, Monochromators, Optical Filters, Photomultiplier Tubes, Polarizers, Beer-Lambart Law, Paraxial Ray Optics and System Designing, Wave Optics, Electromagnetic Theory, Fluorescence Microscopy Systems, Molecular Physics, Photophysics and Stern-Volmer Equation, Jablonski Diagram, Emission Spectra, Fluorescence Lifetime and Quantum Yield, Time-Domain Lifetime Measurements, Fluorescence Correlation Spectroscopy, Total Internal Reflection Fluorescence Microscopy, Electric Field Effects, Point Spread Function, Single- and Multi- Photon Fluorescence Microscopy, Advanced Super Resolution Microscopy, Aperture Engineering Techniques, 3D Image Reconstruction, Markov Random Field, Maximum Likelihood Algorithm, Bayes Theorem. Dedicated Lab Sessions & Practicals on General, Light Sheet and Single Molecule Fluorescence Microscopy.

IN302: Classical & Quantum Optics (Basics) (Aug.-Dec.)

Abstract:

Ray Optics, Matrix Optics, Wave Optics, Diffraction, Interference, Fourier Optics,  Electromagnetic Optics, Polarization Optics, Waves and Particles, Wave Particle Duality / Heisenberg’s Uncertainty Principle, Mathematical Tools for Quantum Optics, Postulates of Quantum Mechanics/Optics, Quantum Harmonic Oscillator, Quantization of Electromagnetic Field, Quantum Theory of Radiation, Quantum States of Light (Coherent and Squeezed light), Bunching, Unbunching and Antibunching, Quantum Distribution Theory (Coherent State Distribution, Wigner Distribution and others), Representation Theory of Light, Atom Field Interaction, Casimir Effect, Hanle Effect, Quantum Lithography, Quantum Microscopy