Optics and Microfluidics Instrumentation (OMI) Lab

Indigenous development of affordable Flow Cytometers & Imaging Flow Cytometers; Non-contact 3-D shape measurement of Cells; Affordable High-Speed Optical Biochemical Assay Systems; Lab to Point of Need Technologies.

Opto-fluidics and Biomedical Instrumentation is an interdisciplinary field integrating optics, microfluidics, and sensor technologies to revolutionize diagnostics and cellular analysis. It focuses on developing high-performance, affordable platforms, including Flow Cytometers, Imaging Flow cytometers and rapid optical bioassays, to enhance the speed, precision, and accessibility of biomedical diagnostics, critically bridging advanced laboratory capabilities with practical solutions for decentralized healthcare settings thereby opening new paradigm of diagnostics. .

Point-of-Care Diagnostics, Lab-on-a-Chip, Imaging Flow Cytometry, High-throughput Phenotyping and Sorting, Disposable Microfluidics

The term lab-on-chip (LOC) refers to the miniaturization of one or several highly sophisticated laboratory process into a single chip (few centimeters in size). The ability to perform complicated reactions/assays on a single chip has enabled the creation of point-of care diagnostic devices. LOC based organ-on-chip devices have enabled the study of behavioral response of organs to certain drugs.

Interferometry, Digital Holography, Fringe Projection Analysis, Digital Holographic Microscopy, Quantitative Phase Imaging, Non-destructive Testing, Time Evolution Studies, Fiber-optic Sensors

Optical metrology encompasses a wide range of non-contact optical measurement techniques like digital holography, interferometry, Fringe projection etc. The use of these techniques has become indispensable to a wide range of industries like aerospace (non-destructive testing and evaluation), microelectronics, medical imaging. These techniques are capable of identifying and measuring defects with sub-micron resolution.

Computational Imaging, Video analysis for Cytometry, Fringe Analysis, Deconvolution, Super-resolution, Real-time Processing

Imaging flow cytometry combines high statistical power of standard flow cytometry with the spatial resolution and quantitative morphology of digital microscopy. With the application of this technique it is possible to image up to a million cells within few minutes. When used in conjunction with computational imaging, flow cytometry can not only capture the 2D morphology of cells, but also reveal multitude of cellular parameters like 3D morphology etc.

Nanotechnology, Microfluidics, Chemical sensing, Surface functionalization, Size controlled synthesis.

Microfluidic Nanotechnology enhances the potentials of nanotechnology with the augmentation of microfluidics. The technology helps in precise size-controlled synthesis of various organic and in-organic nanoparticles which has manifold of applications like microliter chemical analyte sensing.